Catalogue

Background
The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.

Source
Mice were immunized with highly purified monoclonal IgG subclasses isolated from pooled human serum.

Product
Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. 5 Vials with 0.5 ml lyophilized ascites each of the following monoclonal antibodies: MAHu/Igc clones N I 335 and NI 334 MAHu/IgAc clones NI 69 (A89-034) and NI 184 (A89-035) MAHu/IgM clone NI 179 MAHu/IgD clone NI 158 MAHu/IgE clone NI 307

Formulation: Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added.

Concentration: Monoclonal antibody concentration in the reconstituted solution is 1.0 mg/ml. No foreign proteins added.

Specificity
Monoclonal Mouse ascites set containing antibodies to Human IgG, IgA, IgM, IgD and IgE isotype specific.

Species Reactivity: The antibodies do not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species.

Applications
Indirect immunofluorescence,ELISA. Intended use: To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immuno¬fluorescence staining of cytoplasmic Ig, subclasses and immuno¬blotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.

Storage
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day. Monoclonal antibodies should not be stored at a temperature below -25°C due to the aggregation effect of the protein.

Shipping Conditions: Ship at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148